What tubes can be centrifuged with the Centrifuge 5427 R?

Due to the different rotors and adapters, a wide variety of tubes can be centrifuged with the Centrifuge 5427 R: • Micro test tubes (0.5 - 5 mL) • Cryo-Vials • HPLC-Vials • PCR strips and 0.2 mL PCR tubes • Spin columns (1.5/2.0 mL) • Microtainer (0.6 mL)

How can the Centrifuge 5427 R be cleaned?

The Centrifuge 5427 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.

What is the lowest temperature that can be reached with the Centrifuge 5427 R?

The lowest temperature of the Centrifuge 5427 R is -11 °C. The device is thereby optimized with all rotors in such a way that at least 4 °C is maintained with all rotors at the appropriate maximum speeds.

Can the Centrifuge 5427 R automatically recognize an installed rotor?

The Centrifuge 5427 R has an automatic rotor recognition system. A newly installed rotor is recognized at the beginning of the run. The maximum speed of each rotor is automatically limited, if necessary, to prevent over-speeding of the rotors.

In which rotors can I centrifuge 0.5 mL micro test tubes?

0.5 mL micro test tubes can be centrifuged in all rotors for 1.5/ 2.0 mL micro test tubes using an adapter.

Why are the standard rotors in the micro centrifuges 5418 R, 5420, 5424 R, 5425, 5427 R, 5430 and 5430 R aerosol-tight?

At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.

When does the continuous cooling of the rotor chamber start?

The continuous cooling starts with each rotor standstill of the Centrifuge 5427 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met: - The centrifuge is switched on. - The centrifuge lid is closed. - The nominal temperature is lower than the ambient temperature. - The centrifuge is not in standby mode. Continuous cooling is ended by opening the centrifuge lid or by pressing any key.

What benefits do I gain from the centrifugation of my samples with the swing bucket rotor?

Swing-bucket rotors are particularly advantageous for gradient centrifugation applications as horizontal layers are formed that remain in this position at the end of centrifugation (e.g. blood/Ficoll).

Can the Centrifuge 5427 R be opened in the event of a power failure?

Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic cap on the right front corner of the 5427 R has to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.

The "Fast Temp" function is a temperature control run of the Centrifuge 5427 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. The display shows the current temperature and the g-force (rcf) / rotational speed (rpm) as well as "FT" as symbol for a "Fast Temp" temperature run. You can find a more detailed description in the operating instructions of the Centrifuge 5427 R.

What is the benefit of a so-called Kit rotor?

When working with nucleic acid purification kits with spin columns by certain manufacturers, sometimes tube lids cannot be closed any more. This means you have to centrifuge with open tube lids. These tube lids might rip off during the stress of centrifugation and might damage the centrifuge. In order to prevent this, the kit rotor with the raised rotor rim was designed. Open tube lids, supported against the rim, will not rip off during centrifugation. Another feature of this rotor is the enlarged distance between the rotor bores. The enlarged distance between the bores offers a very convenient handling when loading / unloading the rotor. Especially when working with long spin columns (e. g. from Princeton Separations) you will need a larger distance between the bores to load the rotor fully and make use of the complete rotor capacity.

For what applications are aerosol-tight rotors important?

Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.

What is the meaning of the red knob on the rotor lid?

A fixed-angle rotor with a red knob on the rotor lid indicates aerosol-tightness.

In which rotors can I centrifuge 0.2 mL PCR tubes?

Up to 48 0.2 mL PCR tubes (or 6 x 8-tube PCR strips) can be centrifuged in the PCR rotor F-45-48-5-PCR. Furthermore, 0.2 mL PCR tubes can be used in each rotor for 1.5/ 2.0 mL micro test tubes using an adapter

In which rotors can I centrifuge 5 mL micro test tubes?

Up to 16x 5 mL micro test tubes can be centrifuged in the aerosol-tight rotor FA-45-12-17.

Sometimes my Centrifuge 5427 R displays "EP". What does that mean?"

EP" is displayed when the automatic standby function is active. If the centrifuge remains unused for more than 15 min., the standby function is activated, indicated by "EP" on the display. By using any key, the centrifuge is re-activated and ready to use.

Does the Centrifuge 5427 R has an energy saving function?

Yes, with "ECO shut-off" the continuous cooling is switched off if the centrifuge is not used for longer than the preset time. With the default settings, the continuous cooling ends after 8h. In the menu setting "TEMP" the continuous cooling can be limited to 1h, 2h or 4h.

How often does my centrifuge need to be serviced?

This depends on how intensively it is used and varies depending on the load on the device. However, it is recommended to have the centrifuge serviced at least once a year.

What is IQ or OQ and what do I need them for?

Installation and operational qualification; the IQ documents that the device has been delivered and installed correctly. The OQ checks the correct functioning of a device. IQ/OQ ensure that your quality management requirements are met and that your device operates properly and in accordance with the manufacturer’s specifications.

Please contact your local Eppendorf Service Team. You find the contact data on our homepage or in the information materials.

Is the rotor inspection part of the service programs?

No, it can be booked in addition to service programs and depends on the type of rotor (fixed-angle rotor or swing-bucket rotor).

How often does the OQ need to be carried out for a centrifuge?

The company is responsible for technical checks of devices. Eppendorf recommends regular device qualifications depending on the intensity of use, however, they should be carried out at least once a year.

How much are the different centrifuge service programs?

The cost depends on the model and on the service that is performed. Please contact your local Eppendorf Service.

Why should I have my centrifuge serviced at regular intervals?

To ensure the optimum performance and precision of your device. This also enables the early detection of any defects which can then be corrected easily. Performing a large number of cycles increases the susceptibility to rotor damage. Any damage can be detected at an early stage during a qualified inspection, i.e. the rotor can be replaced before any serious damage occurs.

What types of training or online training do you offer to enable me to improve my skills?

Please visit the Eppendorf webpage about the Eppendorf Training Center or contact your local Eppendorf Service for further information and a quotation.

Can I carry out software updates myself?

No. Software updates for centrifuges with a USB interface can be carried out easily by a qualified Eppendorf Service technician via the existing USB port. This ensures that safety factors are up-to-date, that the latest rotors are detected and the running of the machine is optimized with the latest parameters.

How can I clean my centrifuges myself?

Use a neutral cleaning agent for cleaning. We recommend using alcohol or alcohol-based disinfectants for disinfection. Please ensure that the applicable legal regulations and guidelines are observed when selecting the disinfection method. You can find a detailed description in the video "Centrifuge Maintenance - The basis for your lab safety" or in the operating manual which can be downloaded from our website.

Shall we close the lid of the refrigerated centrifuge after the centrifugation is finished?

You should close the lid, if using the centrifuge frequently at lower temperatures. When not in use (e.g. over night) switch off the centrifuge and clean the rotor chamber. The lid should stay open to dry.

What do you need to look out for before carrying out a service?

The device needs to be decontaminated first. The device must be freely accessible and all samples removed from the device.

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Centrifuge 5427 R - 微量遠心機

Centrifuge rotor FA-45-48-11 on the Centrifuge 5427 R

Keypad control on the microcentrifuge Centrifuge 5427 R

The microcentrifuge Centrifuge 5427 R’s OptiBowl® design

Top view of Eppendorf microcentrifuge Centrifuge 5427 R

Soft-touch lid closure on the Centrifuge 5427 R

Aerosol-tight QuickLock® centrifuge rotor lid comes as standard with the Centrifuge 5427 R

Microcentrifuge Centrifuge 5427 R with ergonomic lid design

Streamline your high-end research applications with microcentrifuge Centrifuge 5427 R

製品情報

EppendorfCentrifuge 5427 Rは、高処理能力で研究用の高速冷蔵式微量遠心機です。安全性と信頼性を維持しながら、48本架の2列ローターにより、標準の24本架の微量遠心機と比較してチューブ容量が2倍になります。

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1 製品

Centrifuge 5427 R

ダイヤル, 冷却付, ローターなし
100 V/50 – 60 Hz (JP)

製品のさらなる情報

カタログ番号 5409000039

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お客様のショッピングカートに追加されました。

製品情報

テクニカルデータ

特徴

Centrifuge 5427 Rは高処理能力で研究用途を考慮してデザインされました。 48本架のエアロゾル密閉ローターによって、最も難しい課題でも安全で信頼できる方法で処理し、効率面で必要な性能アップをもたらします。エアロゾル気密の全ロータにはEppendorf QuickLock^® 容易なロータリッドの開閉に対応するリッドが装備されています。 24本架の微量遠心機がチューブ容量に関してニーズを満たさない場合、Centrifuge 5427 Rまたが提供できる、さらなる多用途性が必要ない場合、Centrifuge 5430 Rを選択してください。

ソフトタッチでカバーを閉じるエルゴノミック使用

簡単かつ安全な取り扱い

エアロゾル密閉のEppendorf QuickLockローターはわずか4分の1回転で閉じます。これは日々の使用中の時間を節約するだけでなく、繰り返しによる手首へのストレスをかけません。

Eppendorfチューブ用高速–スイングバケットローター

最大16,050 x gの速度で24 x 1.5 mLまたは2 mLチューブ用の独自のスイングバケットローターを使用して、グラジエント遠心を実行するか、コンパクトなペレットを生成します。エアロゾル密閉のQuickLockローターリッドのおかげで、危険なサンプルを安全かつ簡単に遠心できます。

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Eppendorf製品を登録すると、次の特典があることをご存知ですか？
• 100 epPoints^®の引換（登録した製品ごと）は、リワードショップで利用できます
• 遠心機、シェーカー、CO2インキュベーター、またはサイクラーを登録すると、3か月の延長保証が得られます
• Eppendorf限定の抽選へのエントリー（登録した製品ごとに）
• EppendorfのWebに投稿されるあなたの個人的な製品ストーリーを共有してください。

始めるのはとても簡単です！方法：
1. Eppendorf Appをダウンロードし、スキャンして登録します。
2. 弊社のウェブページにアクセスし、myEppendorfアカウントにログオンして、製品のシリアル番号を入力します
詳細は次をご参照ください：www.eppendorf.com/productregistration

End-of-Life

（寿命の終わり）梱包材の廃棄
フリーザーの梱包材には、木製パレット、段ボール、プラスチック製のダストカバー、発泡部品など、さまざまな素材が使われています。材料のリサイクルは日々ますます重要になっています：ヨーロッパでは、段ボール材料のほぼ100％がリサイクルされていることをご存知ですか？当社のフリーザー包装用段ボールの材料は、約50%リサイクル材料で構成されています。フリーザー段ボールの梱包材も収集し、組織の適切な収集コンテナーに廃棄することにより、貴重な原料をリサイクルするグローバルな持続可能性イニシアチブをサポートしてください。LD-PE製のプラスチック製ダストカバーと発泡部品に関しては、PE材料をリサイクルできる専用のリサイクルパートナーを選択することをお勧めします。利用可能なリサイクルオプションについては、地元の廃棄物処理業者または施設管理チームにお問い合わせいただくことをお勧めします。

装置の廃棄
当社のフリーザーは何年も使用可能ですが、交換が必要になった場合には、地域の要件に従って廃棄するようお願いします。積極的に冷却する装置の経験を持つ、認定された地元のリサイクルパートナーを強くお勧めします。「地元」で処理することは輸送の負荷を軽減します。また、冷却液を安全かつ持続的に除去およびリサイクルすることは、「認証」という点でも推奨されています。

除染
この機器は、ラボで使用されたり、あるいは生物学的試料の取り扱いのために使用されました。処分すべき装置の汚染を適切に除去してください。地域の要件を確認してください。詳細については、地域のバイオセーフティ担当者や廃棄物担当者にお問い合わせください。地域のリサイクル業者に特別な指示や文書化要件があるか確認してください。Eppendorf除染フォームシートを参考として使用できます。

読む量をもっと少なくする

仕様	5427 R

最大 RCF	25000 × g
最大固定角ロータ付きのRCF	25001 × g
最大イングバケットローター付きのRCF	16049 × g
速度	100 – 16,220 rpm
製品タイプ	遠心機
利用可能なローター	9
最大容量	48 × 1.5/2.0 mL
加速時間¹⁾	18 s
減速時間¹⁾	18 s
タイマー	10 秒～9:59 時間
ノイズレベル	< 46 dB（A）（F-45-48-11ローター付き）
電源	100 V, 50 – 60 Hz
最大電力消費	550 W
寸法（W × D × H）	31.9 × 54.0 × 25.4 cm / 12.6 × 21.3 × 10 in
製品の重量	30 kg / 66.1 lb
高さ(蓋開放)	56 cm / 21.9 in
冷却	冷却付
温度範囲	-11 °C ~+40 °C